Kentaro Miyazaki

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Frances ArnoldProfessor of chemical engineering, CaltechVerified email at cheme.caltech.edu
Hikaru SuenagaNational Institute of Advanced Industrial Science and Technology (AIST)Verified email at aist.go.jp
Katsuro YaoiNational Institute of Advanced Industrial Science and Technology (AIST)Verified email at aist.go.jp
Patrick WintrodeUniversity of MarylandVerified email at rx.umaryland.edu
Kei KitaharaOkayama UniversityVerified email at okayama-u.ac.jp
Hidemasa KondoNational Institute of Advanced Technology and Science (AIST)Verified email at aist.go.jp
Sakae TsudaHokkaido University, University of Tokyo, AISTVerified email at alumni.u-tokyo.ac.jp
Yoshiki HiguchiUniversity of HyogoVerified email at sci.u-hyogo.ac.jp
yutaka kawarabayasiNational Institute of Advanced Industrial Science and Technology (AIST)Verified email at aist.go.jp
T. SatyanarayanaNetaji Subhas University of TechnologyVerified email at nsit.ac.in
Nobutaka NakashimaResearcher, AIST JapanVerified email at aist.go.jp
Shinya FushinobuDepartment of Biotechnology, The University of TokyoVerified email at mail.ecc.u-tokyo.ac.jp
Mamoru Suzuki （鈴木　守）大阪大学蛋白質研究所Verified email at protein.osaka-u.ac.jp
Hiroyuki NojiProfessor of Applied Chemistry, Graduate School of Engineering, The University of TokyoVerified email at appchem.t.u-tokyo.ac.jp
Tadashi EguchiTokyo Institute of TechnologyVerified email at chem.titech.ac.jp
DIGVIJAY VERMABabasaheb Bhimrao Ambedkar University, Lucknow, IndiaVerified email at bbau.ac.in
Akihiko YamagishiTokyo University of Pharmacy and Life Science, ProfessorVerified email at toyaku.ac.jp
Hirokazu YanoNational Institute of Infectious DiseasesVerified email at niid.go.jp
Masahiro SotaNAGASE & CO., LTD.Verified email at nagase.co.jp
MASATAKA TSUDATohoku UniversityVerified email at ige.tohoku.ac.jp

Kentaro Miyazaki

Osaka University

Verified email at icb.osaka-u.ac.jp - Homepage

metagenomics synthetic biology ribosome directed evolution


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How enzymes adapt: lessons from directed evolution FH Arnold, PL Wintrode, K Miyazaki, A Gershenson Trends in biochemical sciences 26 (2), 100-106, 2001	556	2001
Functional metagenomics for enzyme discovery: challenges to efficient screening T Uchiyama, K Miyazaki Current opinion in biotechnology 20 (6), 616-622, 2009	446	2009
Directed evolution study of temperature adaptation in a psychrophilic enzyme K Miyazaki, PL Wintrode, RA Grayling, DN Rubingh, FH Arnold Journal of molecular biology 297 (4), 1015-1026, 2000	396	2000
Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function K Miyazaki, FH Arnold Journal of molecular evolution 49, 716-720, 1999	304	1999
A hyperthermophilic laccase from Thermus thermophilus HB27 K Miyazaki Extremophiles 9, 415-425, 2005	260	2005
Creating random mutagenesis libraries using megaprimer PCR of whole plasmid K Miyazaki, M Takenouchi Biotechniques 33 (5), 1033-1038, 2002	222	2002
MEGAWHOP cloning: a method of creating random mutagenesis libraries via megaprimer PCR of whole plasmids K Miyazaki Methods in enzymology 498, 399-406, 2011	210	2011
Functional screening of a metagenomic library for genes involved in microbial degradation of aromatic compounds H Suenaga, T Ohnuki, K Miyazaki Environmental microbiology 9 (9), 2289-2297, 2007	205	2007
Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution PL Wintrode, K Miyazaki, FH Arnold Journal of Biological Chemistry 275 (41), 31635-31640, 2000	183	2000
Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution K Miyazaki, M Takenouchi, H Kondo, N Noro, M Suzuki, S Tsuda Journal of Biological Chemistry 281 (15), 10236-10242, 2006	159	2006
Product-induced gene expression, a product-responsive reporter assay used to screen metagenomic libraries for enzyme-encoding genes T Uchiyama, K Miyazaki Applied and environmental microbiology 76 (21), 7029-7035, 2010	148	2010
Characterization of a novel β-glucosidase from a compost microbial metagenome with strong transglycosylation activity T Uchiyama, K Miyazaki, K Yaoi Journal of Biological Chemistry 288 (25), 18325-18334, 2013	134	2013
Cloning, expression and characteristics of a novel alkalistable and thermostable xylanase encoding gene (Mxyl) retrieved from compost-soil metagenome D Verma, Y Kawarabayasi, K Miyazaki, T Satyanarayana PLoS one 8 (1), e52459, 2013	118	2013
Purification, catalytic properties, and thermal stability of threo-Ds-3-isopropylmalate dehydrogenase coded by leuB gene from an extreme thermophile, Thermus thermophilus … T Yamada, N Akutsu, K Miyazaki, K Kakinuma, M Yoshida, T Oshima The Journal of Biochemistry 108 (3), 449-456, 1990	110	1990
Novel organization of aromatic degradation pathway genes in a microbial community as revealed by metagenomic analysis H Suenaga, Y Koyama, M Miyakoshi, R Miyazaki, H Yano, M Sota, ... The ISME journal 3 (12), 1335-1348, 2009	102	2009
Creating random mutagenesis libraries by megaprimer PCR of whole plasmid (MEGAWHOP) K Miyazaki Directed evolution library creation: methods and protocols, 23-28, 2003	98	2003
Bacterial cellular engineering by genome editing and gene silencing N Nakashima, K Miyazaki International journal of molecular sciences 15 (2), 2773-2793, 2014	91	2014
Metagenomic screening for bleomycin resistance genes T Mori, S Mizuta, H Suenaga, K Miyazaki Applied and environmental microbiology 74 (21), 6803-6805, 2008	91	2008
Revisiting bacterial phylogeny: natural and experimental evidence for horizontal gene transfer of 16S rRNA K Kitahara, K Miyazaki Mobile genetic elements 3 (1), e24210, 2013	86	2013
Patterns of adaptation in a laboratory evolved thermophilic enzyme PL Wintrode, K Miyazaki, FH Arnold Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular …, 2001	78	2001

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